Volume 3 Issue 2

CONTENT LIST

  1. Engineering provitamin A synthesis pathway with β-carotene metabolism in rice endosperm of a restorer line BR827R
  2. Effects of bio-slurry with chemical fertilizer on the performance of some high yielding varieties of boro rice (Oryza sativa L.)
  3. Growth performance of aromatic Boro rice (Oryza sativa L. cv. BRRI dhan50) as influenced by date of transplanting and nutrient management
  4. Effects of nitrite concentrations on soil and certain vegetables irrigated with wastewater of Kubanni stream in Zaria, Kaduna State, Nigeria
  5. Growth of Agathis dammara (Lamb. Rich.) seedling on gold tailing with addition of coconut shell charcoal and compost
  6. Screening of Sesbania accessions based on seed germination and seedling biomass
  7. Phytocomposition and pharmacological importance of Paris polyphylla (Smith.) and needs of its conservation in Arunachal Pradesh, India
  8. Effect of sunlight and artificial light on micropropagation of potato (Solanum tuberosum L.) plantlets
  9. Grain sorghum (Sorghum bicolor L. Moench) fails to consistently respond to N-fertilizer when grown on a Tunica clay soil in the lower Mississippi River Valley, USA
  10. Response surface methodology based optimization of cadmium and lead remediation from aqueous solution by water hyacinth (Eichhornia crassipes [Mart.] Solms) and its anatomical study
  11. Effects of oxytetracycline residues in Thai Koi (Anabas testudineus Bloch) collected from Sylhet, Bangladesh
  12. Response of nitrogen and phosphorus fertilizer rate for sorghum (Sorghum bicolor L. Moench) production in Wag-Lasta area of Ethiopia
  13. Health risk assessment of metals transfer from soil to the edible part of some vegetables grown in Patuakhali province of Bangladesh
  14. Response of okra (Abelmoschus esculentus L. Moench) to vermicompost, mycorrhiza and micronutrients mixture
  15. Effect of integrated nutrient management on growth and productivity of Withania somnifera (L.) Dunal in Kymore Plateau and Satpura hills of Madhya Pradesh, India

Engineering provitamin A synthesis pathway with β-carotene metabolism in rice endosperm of a restorer line BR827R

S. Rehana1*, N. Baishakh2, K. Datta3, N. Oliva4, E. Abrigo4, M.A. Mazid5, Shah-E-Alam6, M.R. Uddin7 and Swapan K. Datta
1Weed Management Laboratory, Department of Agronomy, 1Bio-Technology and Genetic Engineering Discipline, Khulna University, Khulna 9208, BANGLADESH
2School of Plant Environmental and Soil Sciences, Lousiana State University, 214 M.B. Sturgis Hall Baton Rouge, LA 70803, USA
3Laboratory of Translational Research on Transgenic Crops, Department of Botany, University of Calcutta, Kolkata-700073 (West Bengal), INDIA
4Plant Tissue Culture Laboratory, International Rice Research Institute, DAPO 7777, Metro Manilla, PHILIPPINES
5525/A, Senpara Parbata, Mirpur 14, Dhaka 1216, BANGLADESH
6Department of Genetics and Plant Breeding, Bangladesh Agricultural University,   Mymensingh 2202, BANGLADESH
7Department of Agronomy, Bangladesh Agricultural University, Mymensingh 2202, BANGLADESH
8Department of Crop Science, Institute of Agriculture, Visva Bharati University, Santiniketan- 731235 (West Bengal), INDIA
*Corresponding author’s E-mail: [email protected]
Received: 28 April 2018; Revised received: 10 May 2018; Accepted: 15 May 2018

ABSTRACT
Vitamin A deficiency (VAD) is a serious public health problem in South Asia particularly in Bangladesh. Indica rice as a major staple in the country completely lacks vitamin A or compounds with provitamin A activity after milling. A combination of transgenes has been introduced enabling biosynthesis of provitamin A in the endosperm of a restorer line using biolistic system of transformation. The rice seed-specific glutelin promoter (Gt-1 P) was used to drive the expression of phytone synthase (psy), while lycopene b-cyclase (lcy) and phytoenedesaturase (crtI), fused to the transit peptide sequence of the pea-Rubisco small subunit, were driven by the constitutive cauliflower mosaic virus promoter (CaMV35s P). Transgenic plants were recovered through selection with CaMV35sP driven hph (hygromycinphosphotransferase) gene. Molecular analysis demonstrated stable integration and expression of the transgenes. The variable segregation pattern in T1 generation indicated single to multiple insertions of the transgenes in the genome. This is the first report of the development of a transgenic restorer line with carotenogenic pathway into the endosperm for use of hybrid rice improvement.

Keywords: β-carotene, Biolistic transformation, Provitamin A, Restorer line

Effects of bio-slurry with chemical fertilizer on the performance of some high yielding varieties of boro rice (Oryza sativa L.)

M.N. Hossain1, U.K. Sarker1, M.R. Uddin1*, S. Rehana2, M.M.I. Hoque3 and M.A. Islam4
1Department of Agronomy, Bangladesh Agricultural University, Mymensingh-2202, BANGLADESH
2Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna, BANGLADESH
3ACI Limited, Dhaka, BANGLADESH
4Bangladesh Agricultural Development Corporation, Kishoreganj, BANGLADESH
*Corresponding author’s Email: [email protected]
Received: 21 May 2018; Accepted: 30 May 2018

ABSTRACT
Rice yield is greatly influenced by application of manures and fertilizer. Integrated use of organic manure and chemical fertilizer would be quite promising in providing better yield. To evaluate the effect of bio-slurry along with chemical fertilizer, a field experiment was conducted in the Agronomy Field Laboratory, Bangladesh Agricultural University, Mymensingh, Bangladesh. The experiment was comprised of four varieties of boro (dry season irrigated) rice viz., (i) BRRI dhan28, (ii) BRRI dhan29 (iii) Binadhan-8 (iv) Binadhan-10 and four fertilizer management viz., (i) control, (ii) recommended dose of inorganic fertilizer, (iii) [email protected] 5 t ha-1 + inorganic fertilizer, (iv) farmers’ practice (average 15 farmers). The experiment was laid out in a randomized complete block design with three replications. It is evident that variety and fertilizer management had significant effect on effective tillers hill-1, number of grains panicle-1 which ultimately influenced grain yield. The highest grain yield was (6.03 t ha-1) in Binadhan-8 followed by Binadhan-10 and BRRI dhan29. The lowest grain yield was found from BRRI dhan28. In respect of fertilizer management, grain yield was highest (5.90 t ha-1) in bio-slurry @ 5 t/ha + inorganic fertilizer. The lowest grain yield was found from control. The combined effect of variety and fertilizer application showed that highest grain yield (6.10 t ha-1) was found from Binadhan-8 with bio-slurry @ 5 t ha-1 + inorganic fertilizer and the lowest grain yield (4.68 t ha-1) was found from BRRI dhan28 with farmers’ practice. Thus, the variety Binadhan-8 with application of bio-slurry @ 5 t ha-1 + inorganic fertilizer was superior for obtaining highest yield.

Keywords: Bio-slurry, Crop yield, Effective tillers, Fertilizer management, Harvest index, High yield varieties

Growth performance of aromatic Boro rice (Oryza sativa L. cv. BRRI dhan50) as influenced by date of transplanting and nutrient management

Nilufar Yeismin Nila, Swapan Kumar Paul* and Md. Abdur Rahman Sarkar
Department of Agronomy, Bangladesh Agricultural University, Mymensingh-2202, BANGLADESH
*Corresponding author’s E-mail: [email protected]
Received: 13 April 2018; Accepted: 30 May 2018

ABSTRACT
The present experiment  was  conducted  at  the  Agronomy  Field  Laboratory,  Bangladesh Agricultural  University,  Mymensingh,  during  December  2015  to May  2016 to find out the effect of transplanting date and nutrient management on the growth performance of aromatic Boro rice (cv. BRRI dhan50). The experiment comprised five dates of transplanting viz. 15 December, 30 December, 15  January; 30  January and 15  February, and four nutrient managements viz., recommended dose of inorganic fertilizer (N, P, K, S and Zn @ 115, 25, 60, 18, 3.5  kg ha-1,  respectively),  poultry manure @ 5 t ha-1, 25% less than recommended dose of inorganic fertilizer + poultry manure @ 2.5 t ha-1, 50% less than recommended dose of inorganic fertilizer + poultry manure @ 2.5 t ha-1. The experiment was laid out in a randomized complete block design with three replications. Morpho-physiological characteristics were significantly influenced by date of transplanting, nutrient management and their interactions. The highest plant height (71.41 cm) was recorded in early transplanting (15 December) and the lowest plant height (66.29 cm) was recorded when transplanted on 15 February. At 75 DAT, the tallest plant (72.79 cm), the highest number of tillers hill–1 (15.13) and LAI (2.78) were obtained in 25% less than recommended dose of inorganic fertilizers + poultry manure @ 2.5 t ha-1 and the shortest plant (66.63 cm) and lowest  number of tillers hill–1 (11.13) were recorded when applied only poultry manure @ 5 t ha-1. The highest dry matter production hill–1 (31.18 g) at 75 DAT was obtained when the crop was transplanted on 15 December and fertilized with 25% less than recommended dose of inorganic fertilizers + poultry manure @ 2.5 t ha-1.  Therefore, aromatic Boro rice (cv. BRRI dhan50) can be transplanted on 15 December fertilized with 25% less than recommended dose of inorganic fertilizers + poultry manure @ 2.5 t ha-1 for appreciable growth performance.

Keywords: Aromatic Boro rice, Date of transplanting, Growth performance, Morpho-physiological characteristics, Nutrient management

Effects of nitrite concentrations on soil and certain vegetables irrigated with wastewater of Kubanni stream in Zaria, Kaduna State, Nigeria

S.O. Oladeji1*and M.D. Saeed2
1Department of Polymer Technology, Hussaini Adamu Fed. Polytechnic, Kazaure, Jigawa State, NIGERIA
2Department of Chemistry, Bayero University Kano, Kano State, NIGERIA
*Corresponding author’s E-mail: [email protected]
Received: 30 April 2018; Revised received: 09 May 2018; Accepted: 15 May 2018

ABSTRACT
The study examined the effect of nitrite ions in wastewater, soil and vegetables through man-made activities. Nitrite level was determined in wastewater, soil and vegetables viz., spinach (Amaranthus hybridus), lettuce (Lactuca sativa), cabbage (Brassica oleracea), carrot (Daucus carota), okra (Hibiscus esculentus), onion (Allium cepa) and tomato (Lycopersicon esculenetum) collected on seasonal basis along Kubanni stream in Zaria. The levels of nitrate in the wastewater, soils and vegetables were determined using UV/Visible and Smart Spectrophotometer. Results obtained show that nitrite concentrations ranged from 12.05 – 53.21 mg/L in the year 2013 and 1.58 – 17.09 mg/L in the year 2014 for wastewater, soil had concentrations ranged from 0.05 – 6.40 mg/kg in the year 2013 and 0.90 – 9.90 mg/kg for year 2014 while the vegetable had levels of 3.80 – 23.65 mg/kg in the year 2013 and 7.48 – 27.15 mg/kg in the year 2014. Statistical tests indicated no significant difference in nitrite levels across the locations and seasons for wastewater, soil and vegetables evaluated. Correlation results for these two years indicated low (r = 0.399, r = 0.275) relationship for wastewater and vegetables whereas negative (r = -0.290) relationship noticed for the soil. The results showed that irrigating the farmland with untreated wastewater has negative consequence on the crops grown with it and thus, cultivation of wastewater irrigated soils and vegetables should be continuously monitored to stop any possible hazard.

Keywords: ANOVA, Kubanni stream, Nitrite concentration, Soil, Vegetable, Wastewater

Growth of Agathis dammara (Lamb. Rich.) seedling on gold tailing with addition of coconut shell charcoal and compost

Basuki Wasis*, Bayu Winata and Rafika Andriani
Department of Silviculture, Faculty of Forestry, Bogor Agricultural University, Bogor 16680, West Java, INDONESIA
*Corresponding author’s E-mail: [email protected]
Received: 30 April 2018; Revised received: 20 May 2018; Accepted: 28 May 2018

ABSTRACT
Tailing produced by gold mining activities may cause environmental damage, such as decreasing of soil fertility and endangering ecosystem. Tailing also can decrease the soil chacaracteristics and its fertility. Thus, it is not able to carry the plant growth as well. The addition of coconut shell charcoal and compost on tailing may improve the condition of soil properties (physical, chemical, and biological) and its fertility. Agathis dammara (Lamb. Rich.) is one of pioneer tree species that can be potentially used on revegetation process in post mining land. This research was carried out to assess the effect of adding coconut shell charcoal and compost on the growth of A. dammara seedling in tailing medium. This research uses complete random design (CRD) factorial. The result of this research shows that giving  coconut shell charcoal and compost manure  does not have significant effect to all parameter viz height, diameter, total wet weight (TWW), total dry weight (TDW), and root-shoot ratio  (RSR) of A. Dammara. The addition of coconut shell charcoal and compost gave the significant effects on total dry weight (TDW) of A. dammara seedling. The best treatment was the addition of 0 g coconut shell charcoal and 20 g compost (A0B1). Generally, the addition of coconut shell charcoal and compost could improve the soil characteristics and support the growth of the A. dammara seedling on tailing medium.

Keywords: Agathis dammara, Coconut shell charcoal, Compost, Seedling growth, Soil characteristics, Tailing

Screening of Sesbania accessions based on seed germination and seedling biomass

Sontosh C. Chanda*, Nazia Islam, Fardous Jahan Tinne and A.K.M. Golam Sarwar
Laboratory of Plant Systematics, Department of Crop Botany, Bangladesh Agricultural University, Mymensingh 2202, BANGLADESH
*Corresponding author’s E-mail: [email protected]
Received: 13 April 2018; Revised received: 23 May 2018; Accepted: 28 May 2018

ABSTRACT
Seedling emergence and germination percentage of seeds play a vital role for optimum plant population and biomass yield maximization of a crop in the field. An experiment was carried out at Plant Systematics Laboratory of the Department of Crop Botany, Bangladesh Agricultural University, Mymensingh, during the month of April to May 2016 for the screening of Sesbania accessions based on seed germination, vigour index and initial biomass yield. The experiment was laid out in a completely randomized design with four replications. One hundred and ten Sesbania accessions were used as experimental materials. Four hundred healthy seeds, 100 seeds as one replication, of each accession were spread uniformly on containers for the germination test. Cumulative germination percentage of seeds was counted daily up to 10 days. After 10 days, 40 seedlings (10 from each replication) were taken from each accession and measured. Quantitative descriptors viz. emergence (%) and germination (%) of seeds, vigour index, shoot length, root length, base diameter and biomass yield, varied significantly. Based on quantitative descriptors, Agglomerative Hierarchical Clustering (AHC) and Principal Component analyses (PCA) were conducted. In PCA, the first three principal components explained 82.64% of the total variations. In AHC, three individual clusters were developed and six accessions always remained in the same cluster. These six accessions could belong to same species. Based on their seed germination and initial seedling growth, five accessions performed better and selected for further study. Field trials of these accessions are needed to recommend one or more accessions as cultivar(s).

Keywords: Fresh weight and biomass, Germination behavior, Sesbania, Vigour index

Phytocomposition and pharmacological importance of Paris polyphylla (Smith.) and needs of its conservation in Arunachal Pradesh, India

Temin Payum
Jawaharlal Nehru College, Pasighat-East Siang District P.O. Hilltop – 790013 (Arunachal Pradesh), INDIA
E-mail: [email protected]
Received: 22 April 2018; Revised received: 12 May 2018; Accepted: 25 May 2018

ABSTRACT
Paris polyphylla Smith. is a well-known herbal medicine. In Arunachal Pradesh, P. polyphylla has drawn much attention among villagers, print media and the wild life government officials. With the advantage of suitable climate, soil, geographical and weather conditions, this healing herb grows luxuriantly in Arunachal Pradesh but the excessive extraction of this species for illegal trading has resulted into the alarming loss of natural populations. The present study was carried out to study the distribution, population status and chemical compositions of P. polyphylla in Arunachal Pradesh. To answers the objectives of the studies; Interview, Personal observation, field visit and GCMS were used in the study methods. The results of present study reflect unscientific collection, illegal trading, drastic loss of natural population in Arunachal Pradesh; the ethanolic extract gave forty five phytocompounds with numbers of health wellness compounds. Antidepressant, anti uric acid forming compound, anti-cancer, antihypertensive, compounds useful in Parkinson’s disease care and immune system improvement are the major phytocompounds present in P. polyphylla stem. Linolein, 2- mono was found to occupy highest area percentage in TIC peak report with 20.21% while Spirost-5-EN-3-OL, (3.beta. 25R) occupy second highest with 15.31 percent area, respectively. The present study shows need of immediate conservational measures, awareness among the villagers, further phytochemical studies and initiation of propagation to increase the population.

Keywords: Anti-depressant, Gas-chromatography mass spectrometry (GC-MS), Paris polyphylla, Pharmacological importance, Phytocompounds

Effect of sunlight and artificial light on micropropagation of potato (Solanum tuberosum L.) plantlets

S. Rehana1*, F. Ahmed1, N. Zeba2, A. Husna2 and F. Hossain3
1Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna 9208, BANGLADESH
2Department of Genetics and Plant Breeding, Sher-E-Bangla Agricultural University, Agargaon, Dhaka 1207, BANGLADESH
3Rural Development Academy, Bogra, BANGLADESH
*Corresponding author’s E-mail: [email protected]
Received: 18 May 2018; Accepted: 30 May 2018

ABSTRACT
Fluorescent lamps (artificial light) and sunlight were used as lighting source in present study to identify feasibility of using sunlight in plant tissue culture laboratory. In vitro regenerated nodal segments of the cardinal variety of potato (Solanum tuberosum L.) were inoculated in MS medium without any growth regulator for shoot and root development. After 30 days of culture in an average temperature at 21.94±0.21°C, relative humidity 39.35±0.75%, various morphological responses i.e. plantlet height, diameter of plantlet, number of nodes per plantlet, inter node distance, number of leaves per plantlet, fresh and dry weight of plantlets , number of root, length of root, growth rate etc. were recorded. The average light intensity at sunlight and artificial treatment was observed at 4805.5±326.54 lux and 3484±84.44 lux, respectively. All the growth factors performed better result in sunlight treatment than those of artificial one except average number of nodes and leaves. The average height of plantlet at sunlight was 53.33±3.32 mm which was greater than that of artificial light 51.67±2.15 mm. Fresh weight and dry weight are two important morphological factors for plantlet and in case of fresh weight that was always greater 0.49±0.097 in sunlighted plantlet and 0.21±0.026 in artificial one.  In case of dry weight the average weight 0.08±0.016 was greater in sunlighted plantlet than that of 0.03±0.004 in artificial one. Number of roots, root length and growth rate of the plantlets observed higher in sunlighted plantlets compared to artificial light. Moreover, the sunlighted plantlets were healthier, vigorous and strong which helped plants to establish in net house easily. Considering the cost of electricity consumption, from a laboratory area of 20.47 m2 BD Taka 63% could be saved in each month by using sunlight. As low cost options of energy saving in tissue culture laboratory using sunlight would be feasible and environmentally friendly technology for commercial point of view in Bangladesh.

Keywords: Artificial light, Micro-propagation, Morphological responses, Potato plantlets, Sunlight

Grain sorghum (Sorghum bicolor L. Moench) fails to consistently respond to N-fertilizer when grown on a Tunica clay soil in the lower Mississippi River Valley, USA

H. Arnold Bruns
Research Agronomist, USDA-ARS Crop Production Systems Research Unit, P.O. Box 350, Stoneville, MS 38776, USA
E-mail: [email protected] 
Received: 14 April 2018; Revised received: 22 April 2018; Accepted: 04 May 2018

ABSTRACT
Information on producing grain sorghum (Sorghum bicolor L. Moench) on clay soils found in the Mississippi Delta and similar regions is comparatively limited to what is known for the crop in other environments, especially regarding nitrogen fertility. A study conducted in 2014 and 2015 on a Tunica clay soil (clayey over loamy, montmorillonitic, non-acid, thermic, Vertic Halaquept) examined the effects of three N-fertilizer rates (0.0, 112.0, and 224.0 kg N ha-1) on yield and yield components of six commercially available hybrids. The 2014 seeding did not require irrigation while three irrigations were applied in 2015. No yield differences in hybrids or N-fertility treatments were observed in 2014 likely due to waterlogged soil, resulting in denitrification, but added N did increase yields in 2015. No consistent differences in yield or yield components occurred between hybrids either year. Yields in 2014 and at 0.0 or 112.0 kg ha-1 added N-fertilizer in 2015 were sub-standard to regional variety trial data. Rates of N-fertilizer of at least 224.0 kg N ha-1 appear necessary for grain sorghum grown on clay soils in the humid sub-tropics.

Keywords: Irrigation, Sorghum (Sorghum bicolor), Tunica clay soil, Yield, Yield components

Response surface methodology based optimization of cadmium and lead remediation from aqueous solution by water hyacinth (Eichhornia crassipes [Mart.] Solms) and its anatomical study

Vinod Kumar*, Jogendra Singh and Pankaj Kumar
Agro-ecology and Pollution Research Laboratory, Department of Zoology and Environmental Science, Gurukula Kangri Vishwavidyalyaya, Haridwar- 249404, Uttarakhand, INDIA
*Corresponding author’s E-mail: [email protected]
Received: 11 April 2018; Revised received: 23 May 2018; Accepted: 28 May 2018

ABSTRACT
This experiment was performed to optimize the response surface methodology (RSM) based reduction of cadmium (Cd2+) and lead (Pb2+) from the aqueous solution and to study anatomical effects of Cd2+ and Pb2+ stress on stomata of water hyacinth (Eichhornia crassipes [Mart.] Solms) during phytoremediation. Laboratory experiments were carried out to grow E. crassipes plants in six treatments of Cd2+ and Pb2+ viz., 0 mgL-1 (Control), 2 mgL-1, 4 mgL-1, 6 mgL-1, 8 mgL-1 and 10 mgL-1 in 25 liter capacity glass aquariums. A 2-factor central composite design (CCD) with total 25 experimental runs and the predictor regression model equation was applied to optimize the prime conditions for the Cd2+ and Pb2+ reduction. Different plant growth attributes viz., translocation factor; kinetic plant growth rate, fresh plant biomass and total chlorophyll content were also found highest up to 4 mgL-1 concentration of Cd2+ and Pb2+. Structural damage in the stomata of E. crassipes was evaluated under microscopic view and found that above 4 mgL-1 concentration of Cd2+ and Pb2+ in the medium, significant structural damage to the stomata of leaves of the E. crassipes occurred. The results of this study concluded that E. crassipes can remediate Cd2+ and Pb2+ from the medium more efficiently at 1.22 mgL-1 concentration and the developed model can be used to navigate the design space. Furthermore, the different plant growth attributes were also affected above 4 mgL-1 concentration of Cd2+ and Pb2+ in the medium.

Keywords: E. crassipes, Heavy metal toxicity, Phytoremediation, Reduction efficiency, RSM, Stomata damage

Effects of oxytetracycline residues in Thai Koi (Anabas testudineus Bloch) collected from Sylhet, Bangladesh

A.K. Apurbo Barman1, Md. Motaher Hossain1, Md. Golam Rasul2*, Bhaskar Chandra Majumdar2 and Md. Matiur Rahim3
1Department of Fisheries Technology and Quality Control, Sylhet Agricultural University, Sylhet, BANGLADESH
2Department of Fisheries Technology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, BANGLADESH
3Institute of Food science and Technology, Bangladesh Council of Scientific and Industrial Research, Dhaka, BANGLADESH
*Corresponding author’s E-mail: [email protected]
Received: 23 March 2018; Revised received: 16 April 2018; Accepted: 04 May 2018

ABSTRACT
The present study was conducted to determine the levels of oxytetracycline residues in Thai Koi (Anabas testudineus Bloch) collected from some local fish markets of Sylhet Sadar Upazilla, Bangladesh during March to August, 2016. For the purpose of the study, 24 fresh Thai Koi fish samples were randomly collected from four (4) local markets of Sylhet Sadar Upazilla. Concentration of oxytetracycline residues in collected fish samples were determined by High Performance Liquid Chromatography (HPLC) at the Food Toxicology Laboratory of Institute of Food Science and Technology, Bangladesh Council of Scientific and Industrial Research, Dhaka. The results showed that 9 (37.50%) samples with detectable amount of oxytetracycline residues (mean = 42.30±3.00 ppb) were found in Thai Koi. Oxytetracycline residues level below detection limit was also reported in 15 (62.50%) samples of Thai Koi during this study. The detected residues of oxytetracycline in this fish sample did not exceed the maximum residue limit (MRL) 100 ppb recommended by the European Commission. Though residue level of oxytetracycline is lower than the MRL but long term exposure could be hazardous for human health. For that reason, control of antibiotic usage in aquaculture, regular residue monitoring, legislations and regulations for the use of antimicrobials in aquaculture and food safety education are utmost things in this regard.

Keywords: Anabas testudineus, High Performance Liquid, Chromatography, Oxytetracycline, Recommended level

Response of nitrogen and phosphorus fertilizer rate for sorghum (Sorghum bicolor L. Moench) production in Wag-Lasta area of Ethiopia

Workat Sebnie* and Merse Mengesha
Sekota Dry-Land Agricultural Research Center, P.O. Box 62, Sekota, ETHIOPIA
*Corresponding author’s E-mail [email protected]
Received: 09 March 2018; Revised received: 03 May 2018; Accepted: 20 May 2018

ABSTRACT
A field experiment was carried out at Lasta and Sekota woreda Eastern Amhara Ethiopia to assess the effects of application of different rates of N-P fertilizers on yield and yield components of sorghum (Sorghum bicolor). Four rates of N (0, 23, 46, 69, kg ha-1) and three rates of P2O5 (0, 23, 46, kg P2O5 ha-1) were arranged in randomized complete block design (RCBD) with three replications in a factorial arrangement. Nitrogen and phosphorus showed significant effects on yield and yield components of sorghum. Application of nitrogen and phosphorus at a rate of 46 N and 23 kg ha-1 increases the yield of sorghum by about 60.37% at Aybra and 56.33% at Lalibela compared to with control. The highest and profitable yield 3822 kg ha-1 and 2663.28 kg ha-1 was obtained from application of nitrogen and phosphorus at the rate 23 and 23 kg ha-1 and 23 and 46 kg ha-1 at Lalibella and Aybra, respectively. Therefore, application of 46 kg P2O5 and 23 N ha-1 could be appropriate for sorghum production Sekota (Aybra) sorghum growing areas. However, application of 23 N kg ha-1 and 23 P2O5/ha could be appropriate for Lalibella (Shumsha) areas. The current study confirmed that application of fertilizer is very important to boost the production and productivity of sorghum (S. bicolor) in these areas of Ethiopia.

Keywords: Biomass and grain yield, Cultivation budget analysis, Nitrogen and phosphorus fertilizer, Plant height, Sorghum

Health risk assessment of metals transfer from soil to the edible part of some vegetables grown in Patuakhali province of Bangladesh

Md. Saiful Islam1*, Mst. Salma Khanam2, Nazirul Islam Sarker3
1Department of Soil Science, Patuakhali Science and Technology University, Dumki, Patuakhali-8602, BANGLADESH
2Department of Agronomy, Patuakhali Science and Technology University, Dumki, Patuakhali-8602, BANGLADESH
3School of Public Administration, Sichuan University, Chengdu, CHINA
*Corresponding author’s E-mail: [email protected]
Received: 13 April 2018; Revised received: 28 May 2018; Accepted: 31 May 2018

ABSTRACT
This study was conducted to investigate the contamination and chemical speciation of six heavy metals like Cr, Ni, Cu, As, Cd and Pb in soil, their transfer to the edible parts of vegetables i.e. Brinjal (Solanum melongena), Green amaranth (Amaranthus hybridus), Red amaranth (Amaranthus Gangeticus), Bottle gourd (Lagenaria siceraria), Tomato (Solanum lycopersicum), Pumpkin (Cucurbita maxima), Chili (Capsicum annuum L), Carrot (Daucus carota), Bean (Phaseolus vulgaris), Onion (Allium cepa), Potato (Solanum tuberosum) and Lentil (Lens culinaris). The ranges of heavy metals in soil were 3.7-41, 3.9-36, 7.6-46, 2.3-26, 0.61-13 and 4.5-32 mg/kg for Cr, Ni, Cu, As, Cd and Pb, respectively. The metals were mainly associated with the residual fractions of 39%, 41%, 40%, 40%, 34% and 41% for Cr, Ni, Cu, As, Cd and Pb, respectively. In the edible tissues of vegetables, the concentrations of As, Cd and Pb in most vegetable samples exceeded the maximum permissible levels, indicating not safe for human consumption. Target hazard index (HI) value indicates people would experience health risk due to consumption of vegetables. The carcinogenic risk (TR) of As and Pb through consumption of vegetables were higher than the USEPA threshold level (10−6), indicating potential cancer risks.

Keywords: Heavy metals, Health risk, Sequential extraction, Soils, Transfer factor, Vegetables

Response of okra (Abelmoschus esculentus L. Moench) to vermicompost, mycorrhiza and micronutrients mixture

Manisha Dhayal1*, Kavita Aravindkshan1, Manoj Kumar Meena1, Ashok Choudhary2 and Rajesh Choudhary3
1Department of Vegetable science, 2Department of floriculture, College of Horticulture and Forestry, Jhalarapatan city,
Jhalawar-326023 (Rajasthan), INDIA,
3Department of Horticulture, Banaras Hindu University, Varanasi – (Uttar Pradesh), INDIA
*Corresponding author’s E-mail: [email protected]
Received: 23 March 2018; Revised received: 13 April 2018; Accepted: 05 May 2018

ABSTRACT
A field experiments on okra (Abelmoschus esculentus L Moench) cv. Arka Anamika” was conducted at Department of Vegetable Science, College of Horticulture and Forestry, Jhalrapatan city, Jhalawar during the Kharif 2016-17 to study the effect of vermicompost, mycorrhiza and micronutrients mixture of 16 treatment combination i.e. vermicompost with two levels (2 tons/ha and 4 tons /ha), mycorrhiza with one level (Mycorrhiza seed treatment) and micronutrients mixture with two levels (50 ppm and 100 ppm). The observations revealed that the plant height of A. esculentus after 60 DAS (74 cm), plant height after 90 DAS (79.71cm), number of leaves / plant (43.17), main steam diameter (5.52 cm), number of primary branches per plant (5.10) of A. esculentus and lowest days to first flower bud emergence (33.53) of A. esculentus was noted maximum as compared to control. The results indicates that plant height, number of leaves per plant, main stem diameter, stem girth, number of primary branches increased significantly due to application of different levels of vermicompost, mycorrhiza and micronutrients mixture as compared to control. It is postulated that the vermicompost 2tons and 4tons + mycorrhiza seed treatment + micronutrients mixture 50ppm and 100ppm may positively regulate the A. esculentus growth improved. Therefore, application of vermicompost, mycorrhiza along with a mixture of micronutrients played a significant role on enhancing the growth of okra (A. esculentus) and can be applied to obtain the maximum crop yield of okra (A. esculentus).

Keywords: Arka Anamika, Micronutrients mixture, Mycorrhiza, Okra (Abelmoschus esculentus), Vermicompost

Effect of integrated nutrient management on growth and productivity of Withania somnifera (L.) Dunal in Kymore Plateau and Satpura hills of Madhya Pradesh, India

Atul Kumar Shrivastava*, V.B. Upadhyay, D.S. Gautam, S. Sarvade and R.K. Sahu
College of Agriculture, Balaghat, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur-482 004, (Madhya Pradesh), INDIA
*Corresponding author’s E-mail: [email protected]
Received: 03 April 2018; Revised received: 25 April 2018; Accepted: 05 May 2018

ABSTRACT
The field experiment was carried out at the Dusty Acres Farm, Jawaharlal Nehru Krishi Vishwa Vidyalaya (JNKVV), Jabalpur, Madhya Pradesh to study the effect of integrated nutrient management for growth and yield of Withania somnifera (L.) Dunal (Variety: Jawahar Ashwagandha-134). Tallest plants (49.35 cm) were observed at harvest stage, whereas higher number of branches per plant (5.78) of W. somnifera was observed at 90 DAS with T11, respectively. The mean number of leaves per plant (125.40) and LAI (10.345) of W. somnifera was higher at 90 DAS with T11. Mean CGR was maximum (2.536 g m-2 week-1) and mean RGR was highest (0.098 gg-1 day-1) of W. somnifera at 90 DAS and thereafter decline at 120 DAS and harvest stage with T11. Whereas, mean dry matter (1392.60 kg/ha) production of W. somnifera was higher at harvesting stage of crop with T11. Significantly higher mean root length (16.30 cm), root girth (2.26 cm) and mean dry root yield (612.8 kg/ha) of W. somnifera was recorded under T11 than the rest of treatments. Mean number of berries per plant (30.78) and mean number of seeds per berry (194.17) of W. somnifera were significantly higher under T11. Higher mean seed yield (62.6 kg/ha) and harvest index (43.61%) of W. somnifera was observed under T11 as compared to other treatments. Therefore, 100% recommended dose of NPK through fertilizers along with vermicompost (2.5 t/ha), FYM (5.0 t/ha) and ZnSO4 20kg/ha (T11) followed by 50% recommended dose of NPK through fertilizers along with vermicompost (2.5 t/ha), FYM (5.0 t/ha) and ZnSO4 20kg/ha (T14) and 100% recommended dose of NPK through fertilizers along with vermicompost (2.5 t/ha) and ZnSO4 20kg/ha (T10) were found to be better integrated nutrient management for the cultivation of W. somnifera.

Keywords: Crop productivity, Integrated nutrient management, Plant attributes, Recommended dose, Withania somnifera (L.)